The Inhibitory Effects on Cytotoxicity and Nitric Oxide (NO) of the Nano-Encapsulated Extraction of Lipid-Soluble Green Tea Leaves.

The purpose of this study was to investigate the bioactive substances and extraction conditions of lipid-soluble green tee extract using supercritical CO extraction from green tea leaves growing in Boseong-gun, Jeollanam-do and to demonstrate cell safety and effects on inflammatory response of the nano-encapsulated lipid-soluble green tee extract. The polyphenol content of green tea extract using supercritical CO₂ extraction was analyzed. The highest polyphenol was extracted in the condition of 400 bar/50 °C. A total of polyphenol content was 2.47 mg/g. The lipid-soluble vitamin content of supercritical extracts of lipid-soluble green tea was analyzed. Beta-carotene, a vitamin A precursor (4.131 mg/100 g) and vitamin E (537.9 mg/100 g) were detected. ECG content was the highest of catechin of lipid-soluble green tea extract. For cell safety and NO inhibition, human skin cell-derived HaCaT cells and RBL-2H3 mast cells did not have cytotoxicity in 1% of the nano-encapsulated lipid-soluble green tea leaf extract. RAW 264.7 cells did not show cytotoxicity in 5%. NO production was significantly decreased in RAW 264.7 cells in a concentration-dependent manner and within the concentration range. Based on the findings, this study suggested the extraction standards and ingredients of polyphenol according to temperature and pressure in extracting green tea leaf ingredients using supercritical CO₂ extraction. This study also objectively demonstrated the safety and anti-inflammatory effect of the nano-encapsulated lipid-soluble green tea leaf extract which is used as functional materials in the cosmetic industry.