Metabolic fingerprints of Serratia liquefaciens under simulated Martian conditions using Biolog GN2 microarrays.

Sci Rep

University of Florida, 505 Odyssey Way, Space Life Sciences Lab, Exploration Park, Merritt Island, FL, 32953, USA.

Published: October 2018

AI Article Synopsis

  • * The study focused on Serratia liquefaciens, which can grow in low pressure, low temperature, and no oxygen environments, revealing distinct changes in its metabolic processes under varying conditions using Biolog GN2 assays.
  • * Results showed that S. liquefaciens' ability to utilize carbon sources diminished significantly at 0.7 kPa, indicating changes in its physiology that could inform our understanding of potential habitability on Mars.

Article Abstract

Microorganisms growing at atmospheric pressures of 0.7 kPa may have a significant impact on the search for life on Mars. Data on their nutrient requirements in a simulated Martian environment are required to ascertain both the potential risk of forward contamination and the potential of past or present habitability of Mars. Serratia liquefaciens can grow at concomitant conditions of low pressure, low temperature, and anoxic atmosphere. Changes in the metabolic fingerprint of S. liquefaciens grown under varying physical conditions including diverse atmospheric pressures (0.7 kPa to 101.3 kPa), temperatures (30 °C or 0 °C), and atmospheric gas compositions (Earth or CO) were investigated using Biolog GN2 assays. Distinct patterns for each condition were observed. Above 10 kPa S. liquefaciens performed similar to Earth-normal pressure conditions (101.3 kPa) whereas below 10 kPa shifts in metabolic patterns were observed. The differences indicated a physiological alteration in which S. liquefaciens lost its ability to metabolize the majority of the provided carbon sources at 0.7 kPa with a significant decrease in the oxidation of amino acids. By measuring the physiological responses to different carbon sources we were able to identify nutritional constraints that support cellular replication under simulated shallow Mars subsurface conditions.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200771PMC
http://dx.doi.org/10.1038/s41598-018-33856-3DOI Listing

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