AI Article Synopsis
- - Geranylgeraniol (GGOH) is a safe isoprenoid found in various foods and plays a role in muscle cell development.
- - In experiments with C2C12 mouse muscle cells, GGOH decreased markers linked to muscle wasting and promoted muscle cell differentiation without harming cell growth.
- - The findings suggest that GGOH could potentially support muscle health and regeneration, indicating its useful role in myoblast differentiation.
Article Abstract
Background: Geranylgeraniol (GGOH) is a C20 isoprenoid found in fruits, vegetables, and grains, including rice. As a food substance, GGOH is categorized as 'Generally Recognized as Safe'. GGOH is an intermediate product in the mevalonate pathway and acts as a precursor to geranylgeranyl pyrophosphate.
Materials And Methods: C2C12 mouse myoblasts derived from muscle satellite cells were used. Quantitative reverse-transcriptase polymerase chain reaction, western blotting analysis, and immunocytochemical analysis were performed to respectively assess mRNA expression, protein levels, and the number of myofibers.
Results: GGOH reduced the expression levels of skeletal muscle atrophy-related ubiquitin ligases in myofibers derived from C2C12 cells. GGOH induced myogenic differentiation of C2C12 cells via geranylgeranylation. GGOH did not adversely affect the proliferation of C2C12 cells.
Conclusion: GGOH induces myoblast differentiation in C2C12 cells.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365721 | PMC |
| http://dx.doi.org/10.21873/invivo.11395 | DOI Listing |
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