AI Article Synopsis
- - The envelope glycoprotein (Env) is vital for retroviruses, specifically in how they interact with host cells and transport to the plasma membrane.
- - The study identifies Env primarily within endosomes marked by Rab7a and Rab9a, suggesting a specific pathway for its intracellular trafficking.
- - The proposed model outlines that Env is sent from the trans-Golgi network to Rab7a-positive endosomes, then to Rab9a-positive ones, where it helps anchor immature M-PMV particles for transport to the plasma membrane.
Article Abstract
The envelope glycoprotein (Env) plays a crucial role in the retroviral life cycle by mediating primary interactions with the host cell. As described previously and expanded on in this paper, Env mediates the trafficking of immature Mason-Pfizer monkey virus (M-PMV) particles to the plasma membrane (PM). Using a panel of labeled RabGTPases as endosomal markers, we identified Env mostly in Rab7a- and Rab9a-positive endosomes. Based on an analysis of the transport of recombinant fluorescently labeled M-PMV Gag and Env proteins, we propose a putative mechanism of the intracellular trafficking of M-PMV Env and immature particles. According to this model, a portion of Env is targeted from the trans-Golgi network (TGN) to Rab7a-positive endosomes. It is then transported to Rab9a-positive endosomes and back to the TGN. It is at the Rab9a vesicles where the immature particles may anchor to the membranes of the Env-containing vesicles, preventing Env recycling to the TGN. These Gag-associated vesicles are then transported to the plasma membrane.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212865 | PMC |
| http://dx.doi.org/10.3390/v10100575 | DOI Listing |
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