We investigated the beneficial effects and underlying mechanisms of Zhuanggu Guanjie (ZGGJ) pill in osteoporosis and . Bone marrow macrophages from 4-6-week-old mice were cultured in the presence of macrophage colony-stimulating factor (15 ng/mL) and receptor activator of nuclear factor-B ligand (30 ng/mL). Osteoclast differentiation was determined by quantification of tartrate-resistant acid phosphatase activity. Gelatin zymography was used to detect the activity of matrix metalloproteinases in osteoclasts. Ovariectomized rats were administered orally with estradiol valerate or ZGGJ for 8 weeks. Blood was collected to measure serum indices. Tibiae were harvested to carry out bone microcomputed tomography scanning, histomorphological analysis, and bone strength determination. ZGGJ inhibited tartrate-resistant acid phosphatase activity, matrix metalloproteinase 9 expression, and bone resorption . At doses of 0.55, 1.1, and 2.2 g/kg, ZGGJ exerted significant osteoprotective effects including inhibition of bone turnover markers and improved tibia bone strength in ovariectomized rats. Microcomputed tomographic analysis showed that ZGGJ improved the trabecular architecture with increased connectivity density and trabecular thickness and decreased trabecular spacing. These results revealed that ZGGJ prevents bone loss induced by ovariectomy in rats and that inhibition of bone resorption is involved in the bone-protective effects of ZGGJ.
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| http://dx.doi.org/10.1155/2018/9075318 | DOI Listing |
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Determination of multicomponents from Zhuanggu Guanjie capsule in rat plasma by UHPLC-MS/MS and pharmacokinetic study.
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Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Jinghai District, Tianjin, 301617, China.
To develop a UHPLC-MS/MS method for the quantification of 12 constituents in rat plasma after oral administration of Zhuanggu Guanjie. Constituent separation was performed on a C column, and the mass spectrometric detection was performed in multiple reaction monitoring mode with a positive-negative ionization mode. The method was successfully applied to the pharmacokinetic study of these 12 constituents after rats taking Zhuanggu Guanjie capsules.
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College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, China.
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View Article and Find Full Text PDF[Drying effect of Chinese medicinal pills based on new spiral vibration drying technology].
Zhongguo Zhong Yao Za Zhi
March 2022
Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
Article Synopsis
- - The study investigated the effectiveness of a new spiral vibration drying technology on various Chinese medicinal pills and compared it to traditional methods like hot air and vacuum drying.
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Study on the effects of Zhuanggu Guanjie Pill, a modern Chinese medicine formula, on the activities and mRNA expression of seven CYP isozymes in rats.
J Ethnopharmacol
December 2021
New Drug Screening Center, Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, Nanjing, 210009, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:
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