An electroanalysis strategy has been developed for probing glutathione (GSH) separately in hela and yeast cells based on the displacement reaction route using melamine-copper (MA-Cu) nanocomposites. Herein, MA-Cu nanocomposites were initially synthesized by the controlled supermolecular self-assembly process showing various morphological structures depending on the MA-to-Cu ratios used. It was discovered that the electrodes modified with rod-like MA-Cu nanocomposites could achieve the stable electrochemical output of solid-state CuCl at a low potential, which might circumvent the possible interference from co-existing electroactive substances in complicated backgrounds like cells. More importantly, the yielded CuCl signals would decrease selectively induced by GSH through the specific Cu-GSH interaction that would trigger the displacement of CuCl into non-electroactive complex. The MA-Cu nanorods-modified electrodes can allow for the detection of GSH with the concentrations linearly ranging from 0.010 to 300.0 μM. Subsequently, the feasibility of the developed electroanalysis strategy was demonstrated for the evaluation of GSH separately in the extractions of hela and yeast cells, promising the wide applications in the clinical and food analysis fields.
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http://dx.doi.org/10.1016/j.bios.2018.10.022 | DOI Listing |
ACS Meas Sci Au
December 2024
Department of Physiology, Sahlgrenska Academy, University of Gothenburg, Medicinaregatan 11-13, 41390 Gothenburg, Sweden.
Single cell Amperometry (SCA) is a powerful, sensitive, high temporal resolution electrochemical technique used to quantify secreted molecular messengers from individual cells and vesicles. This technique has been extensively applied to study the process of exocytosis, and it has also been applied, albeit less frequently, to investigate insulin exocytosis from single pancreatic beta cells. Insufficient insulin release can lead to diabetes, a chronic lifestyle disorder that affects millions of people worldwide.
View Article and Find Full Text PDFCrit Rev Anal Chem
November 2024
Department of Chemistry, Federal University of Viçosa, Viçosa, Brazil.
An overview of the latest advances in the design of electrochemical sensor architectures dedicated to the determination of drugs from the statin class is presented in this review. Statins are drugs widely consumed for cholesterol control, and their determination in different matrices through the application of electroanalysis is growing considering advantages such as operational simplicity, lower cost and ease of sample preparation. Within the context of statins, electrochemical sensor architectures can be subdivided into conventional/classical electrodes such as glassy carbon electrodes, carbon paste electrodes, pencil graphite electrodes, boron-doped diamond electrodes and metallic electrodes, and more modern electrode systems, including the screen-printed electrodes and 3D-printed electrodes.
View Article and Find Full Text PDFMikrochim Acta
October 2024
School of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China.
A dual-responsive ratio electrochemical-colorimetric method for nitrite (NO) is established based on the combination of nanoenzyme (MnO) catalysis with diazotization reactions. The MnO can oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue TMBox. The NO induces the diazotization reaction of TMBox, leading to a decrease of the signal at 652 nm and the generation of a new signal from diazotized TMBox at 445 nm.
View Article and Find Full Text PDFAnal Chem
July 2024
Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy.
Lipid nanovectors (LNVs) represent potent and versatile tools in the field of drug delivery for a wide range of medical applications including cancer therapy and vaccines. With this Technical Note, we introduce a novel "portable", easy-to-use, and low-cost strategy for double use: (1) it allows one to both quantify the amount of cargo in LNV formulation and (2) classify the nature of formulation with the aim of chemometrics. In particular, an electrochemical strip, based on a screen-printed electrode, was exploited to detect methylene blue (MB) as the model cargo encapsulated in various liposomes (used as model LNV).
View Article and Find Full Text PDFAnal Bioanal Chem
August 2024
Department of Pharmacy, University of Naples "Federico II", Via Domenico Montesano 49, 80131, Naples, Italy.
In the era of liquid biopsy, microRNAs emerge as promising candidates for the early diagnosis and prognosis of cancer, offering valuable insights into the disease's development. Among all the existing analytical approaches, even if traditional approaches such as the nucleic acid amplification ones have the advantages to be highly sensitive, they cannot be used at the point-of-care, while sensors might be poorly sensitive despite their portability. In order to improve the analytical performance of existing electroanalytical systems, we demonstrate how a simple chromatographic paper-based disk might be useful to rationally improve the sensitivity, depending on the number of preconcentration cycles.
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