Human ALKBH3-induced mA demethylation increases the CSF-1 mRNA stability in breast and ovarian cancer cells.

Biochim Biophys Acta Gene Regul Mech

The University of Arizona Cancer Center, Tucson, AZ 85724, USA; Department of Obstetrics and Gynecology, College of Medicine, The University of Arizona, Tucson, AZ 85724, USA.

Published: January 2019

In ovarian and breast cancers, the actions of the cytokine CSF-1 lead to poor prognosis. CSF-1 expression can be regulated post-transcriptionally. RNA methylation is another layer of posttranscriptional regulation. The methylation of N atom of adenine (mA) results in a conformational change of RNA which regulates translational efficiency. Our study indicates that the mA is also involved in the CSF-1 mRNA decay. The alteration of ALKBH3 expression, an mA demethylase, regulates the CSF-1 mRNA stability. Demethylation of mA by ALKBH3 increases the half-life of CSF-1 mRNA without affecting the translation efficiency. The mA in CSF-1 mRNA is mapped in the 5'UTR near the translation initiation site. YTHDF2, a known mA reader which interacts with the CCR4-NOT deadenylation complex, is not the reader of mA-containing CSF-1 mRNA. Overexpression of ALKBH3 increases CSF-1 expression and the degree of cancer cell invasiveness without affecting cell proliferation or migration. Collectively, we showed that CSF-1 mRNA decay can be regulated at an epigenetic level, and that alteration of the N‑methylation status leads to phenotypic changes in cancer cell behavior.

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Source
http://dx.doi.org/10.1016/j.bbagrm.2018.10.008DOI Listing

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