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Hepatic and pulmonary macrophage activity in a mucosal challenge model of Ebola virus disease.
Front Immunol
December 2024
Department of Pathology, University of Texas Medical Branch, Galveston, TX, United States.
Background: The inflammatory macrophage response contributes to severe Ebola virus disease, with liver and lung injury in humans.
Objective: We sought to further define the activation status of hepatic and pulmonary macrophage populations in Ebola virus disease.
Methods: We compared liver and lung tissue from terminal Ebola virus (EBOV)-infected and uninfected control cynomolgus macaques challenged via the conjunctival route.
Oceanic Breakthroughs: Marine-Derived Innovations in Vaccination, Therapy, and Immune Health.
Vaccines (Basel)
November 2024
Institute of Biostructures and Bioimaging, Italian National Research Council (IBB-CNR), Via P. Castellino 111, 80131 Naples, Italy.
The vast, untapped potential of the world's oceans is revealing groundbreaking advancements in human health and vaccination. Microalgae such as spp. and are emerging as resources for recombinant vaccine development with specific and heterologous genetic tools used to boost production of functional recombinant antigens in and spp.
View Article and Find Full Text PDFDevelopment of Long-Term Stability of Enveloped rVSV Viral Vector Expressing SARS-CoV-2 Antigen Using a DOE-Guided Approach.
Vaccines (Basel)
October 2024
Viral Vectors and Vaccines Bioprocessing Group, Department of Bioengineering, McGill University, Montreal, QC H3A 0E9, Canada.
Liquid formulations have been successfully used in many viral vector vaccines including influenza (Flu), hepatitis B, polio (IPV), Ebola, and COVID-19 vaccines. The main advantage of liquid formulations over lyophilized formulations is that they are cost-effective, as well as easier to manufacture and distribute. However, studies have shown that the liquid formulations of enveloped viral vector vaccines are not stable over extended periods of time.
View Article and Find Full Text PDFQuantification of Filovirus Soluble Glycoprotein Using a Sandwich ELISA.
Methods Mol Biol
November 2024
National Research Center for the Control and Prevention of Infectious Diseases, Nagasaki University, Nagasaki, Japan.
Sandwich enzyme-linked immunosorbent assays (ELISAs), which quantify antigens captured between two layers of antibodies, are sensitive and fundamental tools used to diagnose diseases, evaluate the effects of countermeasures, and quantify target proteins. Many filoviruses use transcriptional editing to express three different glycoproteins (GPs) from the GP gene. The main product transcribed from the GP gene, the soluble glycoprotein (sGP), is detected at high levels in the blood during the acute phase of infection and correlates with viremia, making it an attractive target for molecular diagnostics.
View Article and Find Full Text PDFIn Vivo Investigation of Filovirus Glycoprotein-Mediated Infection in a BSL2 Setting.
Methods Mol Biol
November 2024
Department of Microbiology and Immunology, University of Iowa, Iowa City, IA, USA.
Article Synopsis
- Filoviruses, like Ebola virus (EBOV), cause filovirus disease (FVD) and pose significant global health threats, but studying them has been challenging due to safety restrictions.
- A new research tool involving a modified vesicular stomatitis virus (VSV-filo GP) allows researchers to safely explore the interactions and immune responses related to filovirus infections outside high-security labs.
- The study utilizes interferon α/β receptor-deficient (Ifnar) mice to examine different methods of infection and disease progression, providing a cost-effective and manageable way to advance filovirus research.
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