Supplementary data are provided which are supportive to the research article entitled "Characterization and safety evaluation of HPPD W336, a modified 4-hydroxyphenylpyruvate dioxygenase protein, and the impact of its expression on plant metabolism in herbicide-tolerant MST-FGØ72-2 soybean" (Dreesen et al., 2018) [1]. The conducted supplementary analyses include the characterization of additional -produced HPPD W336 protein batches used as a surrogate in HPPD W336 safety studies, the assessment of potential glycosylation and monitoring of stability in simulated intestinal fluid and during heating of the HPPD W336 protein. Furthermore, data are provided on conducted field trials and subsequent compositional analysis in MST-FGØ72-2 soybean grain of compounds related to the tyrosine degradation pathway and the metabolism of homogentisate.
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http://dx.doi.org/10.1016/j.dib.2018.08.035 | DOI Listing |
Cotton GHB811 was developed to confer tolerance to glyphosate and HPPD inhibitor herbicides. The molecular characterisation data and bioinformatic analyses do not identify issues requiring food/feed safety assessment. None of the identified differences in the agronomic/phenotypic and compositional characteristics tested between cotton GHB811 and its conventional counterpart needs further assessment, except for % lint, lint length and dihydrosterculic acid, which do not raise nutritional and safety concerns.
View Article and Find Full Text PDFData Brief
December 2018
BASF Agricultural Solutions Belgium N.V., Technologiepark-Zwijnaarde 38, B-9052 Gent, Belgium.
Supplementary data are provided which are supportive to the research article entitled "Characterization and safety evaluation of HPPD W336, a modified 4-hydroxyphenylpyruvate dioxygenase protein, and the impact of its expression on plant metabolism in herbicide-tolerant MST-FGØ72-2 soybean" (Dreesen et al., 2018) [1]. The conducted supplementary analyses include the characterization of additional -produced HPPD W336 protein batches used as a surrogate in HPPD W336 safety studies, the assessment of potential glycosylation and monitoring of stability in simulated intestinal fluid and during heating of the HPPD W336 protein.
View Article and Find Full Text PDFRegul Toxicol Pharmacol
August 2018
Bayer CropScience N.V. - Innovation Center, Tech Lane Ghent Science Park 38, B-9052, Gent, Belgium. Electronic address:
By transgenic expression technology, a modified 4-hydroxyphenylpyruvate dioxygenase enzyme (HPPD W336) originating from Pseudomonas fluorescens is expressed in MST-FGØ72-2 soybean to confer tolerance to 4-benzoyl isoxazole and triketone type of herbicides. Characterization and safety assessment of HPPD W336 were performed. No relevant sequence homologies were found with known allergens or toxins.
View Article and Find Full Text PDFRegul Toxicol Pharmacol
April 2018
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, PR China. Electronic address:
The genetically modified (GM) soybean FG72 contains two exogenous genes: p-hydroxyphenylpyruvate dioxygenase (hppd) and double mutant 5-enol pyruvylshikimate-3-phosphate synthase (2mepsps), endowing the FG72 with the glyphosate and isoxaflutole herbicides resistant abilities for presence of the 2mEPSPS and HPPD W336 proteins. A food safety assessment of GM soybean FG72 was evaluated by a 90-days feeding study using three different dietary concentrations (7.5%, 15%, or 30% w/w) of the GM soybean or its corresponding non-GM cultivar Jack fed to Sprague-Dawley rats.
View Article and Find Full Text PDFIn this opinion, the EFSA Panel on Genetically Modified Organisms (GMO) assesses the two-event stack soybean FG72 × A5547-127 for food and feed uses, import and processing. The EFSA GMO Panel previously assessed the two single events combined to produce the two-event stack soybean FG72 × A5547-127 and did not identify safety concerns. No new data on the single events, leading to modification of the original conclusions on their safety, were identified.
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