Background: In the current study, nine foodborne "Locus of Enterocyte Effacement" (LEE)-negative Shiga toxin-producing (STEC) strains were selected for whole genome sequencing and analysis for yet unknown genetic elements within the already known LEE integration sites , and . Foreign DNA ranging in size from 3.4 to 57 kbp was detected and further analyzed. Five STEC strains contained an insertion of foreign DNA adjacent to the tRNA gene and five and seven strains contained foreign DNA adjacent to the and tRNA genes, respectively. We characterized the foreign DNA insertion associated with (STEC O91:H21 strain 17584/1), (STEC O8:H4 strain RF1a and O55:Hnt strain K30) and (STEC O91:H21 strain 17584/1 and O113:H21 strain TS18/08) as examples.
Results: In total, 293 open reading frames partially encoding putative virulence factors such as TonB-dependent receptors, DNA helicases, a hemolysin activator protein precursor, antigen 43, anti-restriction protein KlcA, ShiA, and phosphoethanolamine transferases were detected. A virulence type IV toxin-antitoxin system was detected in three strains. Additionally, the system was found in one strain. In strain 17584/1 we were able to define a new genomic island which we designated GI . The island contained integrases and mobile elements in addition to genes for increased fitness and those playing a putative role in pathogenicity.
Conclusion: The data presented highlight the important role of the three tRNAs , and for the genomic flexibility of .
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| http://dx.doi.org/10.1186/s13099-018-0271-8 | DOI Listing |
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