Rapid and accurate identification of pathogen is a major quarantine strategy for outbreak prevention. We used capillary electrophoresis-random amplified polymorphic DNA (CE-RAPD) to generate highly discriminatory pathogen profiles, reduced batch effects between profiles by novel normalization procedure and pattern of technical repeats, followed by target similarity evaluation using target identification score (TIS). A full target signature contains several patterns. TIS system was optimized by training set isolates that included three species, and validated using two hundred clinical Klebsiella pneumoniae isolates. Hierarchical clustering analysis showed CE-RAPD profiles arrange clusters according to the species or the source. Moreover, samples with similar profile may display similar antibiotic susceptibility. By using a signature of four patterns, the TIS system could accurately identify target among different isolates. The variation between isolates may be caused by small change in genome. TIS system provides a standardized tool for building of outbreak firewall and facilitate data exchange.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193972 | PMC |
| http://dx.doi.org/10.1038/s41598-018-33702-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antibody functionalized curcuma-derived extracellular vesicles loaded with doxorubicin overcome therapy-induced senescence and enhance chemotherapy.
J Control Release
January 2025
Department of General Surgery, Cancer Hospital of Dalian University of Technology, Liaoning Cancer Hospital & Institute, Shenyang, Liaoning 110042, China; Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, China. Electronic address:
Conventional cancer treatments often induce a sustained DNA damage response (DDR) in tumor cells, leading to therapy-induced senescence (TIS), characterized by permanent cell cycle arrest and resistance to apoptosis. These senescent cells secrete senescence-associated secretory phenotypes (SASP), which can promote tumor progression and create an immunosuppressive microenvironment. This study introduces a novel approach to enhance chemotherapy efficacy by using functionalized curcuma-derived extracellular vesicles (DR5-CNV/DOX) to target and eliminate senescent tumor cells and inhibit their SASP.
View Article and Find Full Text PDFTablet diversion strategy based on in-line NIR tablet press feed frame measurements.
Int J Pharm
January 2025
Pharmaceutical Engineering Research Group (PharmaEng), Department of Pharmaceutical Analysis, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. Electronic address:
The tablet diversion strategy, based on in-line near-infrared (NIR) tablet press feed frame measurements, can be a key component of both batch and continuous oral solid dose manufacturing processes. It enables real-time, high-frequency monitoring and control, enhancing process understanding and compliance compared to conventional interval-based sampling methods. Central to this strategy are NIR spectrometers, which serve as PAT systems for in-line blend uniformity monitoring in the feed of the tablet press.
View Article and Find Full Text PDFThe International System for Serous Fluid Cytopathology (TIS) survey in preparation for TIS 2.0.
J Am Soc Cytopathol
December 2024
Department of Cellular Pathology, Guy's & St. Thomas' NHS Foundation Trust, London, UK.
Introduction: The International System for Serous Fluid Cytopathology (TIS) has gained acceptance and has led to literature validating original concepts and suggesting refinements. In preparation for the second edition of TIS, editors generated a survey to solicit experience with and opinions about TIS.
Materials And Methods: An online survey available from March 8 to June 15, 2024, included 56 questions, offered in 7 languages, related to the practice of serous fluid cytopathology.
RITA Temporary Immersion System (TIS) for Biomass Growth Improvement and Ex Situ Conservation of Erben & Raimondo.
Plants (Basel)
December 2024
Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Campus Ecotekne, 73100 Lecce, Italy.
Erben & Raimondo is a rare and endangered taxon, endemic to a limited area on Mount Pizzuta in northwestern Sicily, Italy. Its population is significantly threatened by anthropogenic activities, including fires, overgrazing, and habitat alterations. Temporary immersion systems (TISs) have proven effective for large-scale propagation in various protected species, offering potential for ex situ conservation and population reinforcement of .
View Article and Find Full Text PDFA Leadfield-Free Optimization Framework for Transcranially Applied Electric Currents.
bioRxiv
December 2024
Danish Research Centre for Magnetic Resonance, Department of Radiology and Nuclear Medicine, Copenhagen University Hospital Amager and Hvidovre, Hvidovre, Denmark.
Background: Transcranial Electrical Stimulation (TES), Temporal Interference Stimulation (TIS), Electroconvulsive Therapy (ECT) and Tumor Treating Fields (TTFields) are based on the application of electric current patterns to the brain.
Objective: The optimal electrode positions, shapes and alignments for generating a desired current pattern in the brain vary between persons due to anatomical variability. The aim is to develop a flexible and efficient computational approach to determine individually optimal montages based on electric field simulations.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!