Dissociated neurons from the brain of pteropodial mollusc were cultivated in a 25% Leibovitz medium containing 2% of calf serum. Neurite outgrowth was observed in 1-30% of the neurons. It was maximum during the first 3 days. Neurite length reached 300 microns. Membrane potential of neurons was 40-60 mV; they generated single spikes or bursts of impulses. Intercellular connections were tested on the 3-4th days in 70 pairs of neurons with neurites overlapped. Electrical connections between the cells were observed in 20% of the pairs tested, and in 6% of pairs stimulation of one of the neurons evoked an inhibitory postsynaptic potential in the other.
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[Neural regulation of heart function in the Pteropod mollusc Clione limacina].
Neirofiziologiia
September 1989
The heart of the pteropodial mollusc Clione limacina is innervated by the median nerve arising from the left abdominal ganglion. Five neurons sending axons to the heart have been identified in the Clione central nervous system with retrograde cobalt or Lucifer yellow staining. Neuron H1 located in the left pedal ganglion evoked heart excitation.
View Article and Find Full Text PDFDissociated neurons from the brain of pteropodial mollusc were cultivated in a 25% Leibovitz medium containing 2% of calf serum. Neurite outgrowth was observed in 1-30% of the neurons. It was maximum during the first 3 days.
View Article and Find Full Text PDFIn the pteropodial mollusc Clione limacina, the rhythmic locomotor wing movements are controlled by the pedal ganglia. The locomotor rhythm is generated by two groups of interneurons (groups 7 and 8) which drive efferent neurons. In the present paper, the activity of isolated neurons, which were extracted from the pedal ganglia by means of an intracellular electrode, is described.
View Article and Find Full Text PDFThe isolated pedal ganglia of the pteropodial mollusc Clione limacina generate the locomotor activity. In 30% of the pedal ganglion preparations, the locomotor rhythm was not regular, i. e.
View Article and Find Full Text PDFEfferent neurons in isolated pedal ganglia of the pteropodial mollusc Clione limacina were filled with Lucifer Yellow through the wing nerves. Then the ganglia were illuminated with intense blue light which resulted in the complete inactivation of these neurons. After inactivation of efferent neurons, interneurons of the pedal ganglia continued to generate the locomotor rhythm.
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