Data on the hemolysin synthesis determined by the plasmid pHly241, belonging to the incompatibility group I2, and its derivatives are presented in this paper. The restriction analysis of the pHly241 plasmid has resulted in the detailed mapping of the hly determinant region in the plasmid. The substantial homology has been demonstrated between the regions of the plasmids pHly241 and pHly195 coding for hemolysin synthesis and excretion from the bacterial cell.

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