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Holo-Seq: single-cell sequencing of holo-transcriptome. | LitMetric

Holo-Seq: single-cell sequencing of holo-transcriptome.

Genome Biol

Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, 310058, Zhejiang, People's Republic of China.

Published: October 2018

AI Article Synopsis

Article Abstract

Current single-cell RNA-seq approaches are hindered by preamplification bias, loss of strand of origin information, and the inability to observe small-RNA and mRNA dual transcriptomes. Here, we introduce a single-cell holo-transcriptome sequencing (Holo-Seq) that overcomes all three hurdles. Holo-Seq has the same quantitative accuracy and uniform coverage with a complete strand of origin information as bulk RNA-seq. Most importantly, Holo-Seq can simultaneously observe small RNAs and mRNAs in a single cell. Furthermore, we acquire small RNA and mRNA dual transcriptomes of 32 human hepatocellular carcinoma single cells, which display the genome-wide super-enhancer activity and hepatic neoplasm kinetics of these cells.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193298PMC
http://dx.doi.org/10.1186/s13059-018-1553-7DOI Listing

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