Fatty Acid Composition and Eicosanoid Levels (LTE and PGE) of Human Milk from Normal Weight and Overweight Mothers.

Maternal obesity is known to affect human milk composition. Long-chain polyunsaturated fatty acids (LCPUFA) are vital nutrients to the nervous system development and precursors of eicosanoids related to obesity (prostaglandin E-PGE-and leukotriene E-LTE). The aim of the present research was to study the lipid profiles, with particular emphasis to LCPUFAs, and the concentrations of eicosanoids PGE and LTE, involved in adipose tissue development, in human milk from overweight mothers compared with normal weight mothers. Study including 46 overweight and 86 normal weight breastfeeding volunteers was carried out. Fatty acids and eicosanoids (PGE and LTE) were analyzed in mature human milk. Fatty acids quantification was determined by gas chromatography and mass spectrometry. PGE and LTE were measured by immununoassay. Human milk of overweight mothers had lower contents of n-3 LCPUFA, including eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) and higher levels of total n-6 LCPUFA, compared with normal weight mothers (0.45 ± 0.23 versus 0.58 ± 0.38,  = 0.016; 0.05 ± 0.04 versus 0.08 ± 0.08,  = 0.005; 0.26 ± 0.15 versus 0.34 ± 0.22,  = 0.015; 0.84 ± 0.25 versus 0.74 ± 0.20,  = 0.029; respectively). Multiple regression analyses showed that maternal overweight was associated with human milk fatty acid profile. The levels of PGE and LTE in human milk did not show significant differences between groups. Our findings support the hypothesis that mother weight status influences human milk n-3 LCPUFA lipid composition, but not its relationship with PGE and LTE levels.