To identify precursor cells of xanthophores (xanthoblasts), non-pigmented cells without any phenotypic traits as pigment cells were isolated from the dermal tissue of xanthic goldfish with an adult color pattern and cultured in a medium containing 1 mM db-cAMP or 0.25 U/ml ACTH and 10% carp serum. These non-pigmented cells differentiated into xanthophores which showed a dendritic morphology and contained a large quantity of fluorescent pteridines and numerous vesicular inclusions. Sepiapterin was the major component, and the vesicles contained fuzzy material in addition to small membranous elements. The fluorescent pattern and the morphological characteristics indicated that the differentiated pigment cells were xanthophores of larval type.
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http://dx.doi.org/10.1016/0045-6039(87)90430-1 | DOI Listing |
Histol Histopathol
April 1996
Institut fur Biologie II (Zoologie), RWTH Aachen, Germany.
In this study, melanophore cytodifferentiation in the fins of xanthic goldfish that had been exposed to osmotic stress for 18 days was investigated. It was found that multi-vesicular bodies (MVB) are not the only type of premelanosome. Granules having a homogeneous matrix also function as premelanosomes.
View Article and Find Full Text PDFTo identify precursor cells of xanthophores (xanthoblasts), non-pigmented cells without any phenotypic traits as pigment cells were isolated from the dermal tissue of xanthic goldfish with an adult color pattern and cultured in a medium containing 1 mM db-cAMP or 0.25 U/ml ACTH and 10% carp serum. These non-pigmented cells differentiated into xanthophores which showed a dendritic morphology and contained a large quantity of fluorescent pteridines and numerous vesicular inclusions.
View Article and Find Full Text PDFUltrastructural studies, and cytochemical and biochemical determinations of tyrosinase activity were conducted on the pigment epithelium of albino and xanthic goldfish eyes. In eyes of xanthic goldfish, two types of melanosomes are present, spherical and elongated. Melanized melanosomes are absent in the eyes of the albino goldfish, but elongated lamellar premelanosomes are observed.
View Article and Find Full Text PDFIntegumental erythrophores on the scales of xanthic goldfishes were dissociated and cultured in a monolayer for about 30 days. The proportion of erythrophores in the culture was about 50% at the beginning of cultivation. Erythrophores in culture were characterized by the aggregate of orange-coloured pigment in the centre of the cell body.
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