AI Article Synopsis
- * These cells showed a slow doubling time of 114.34 hours and have a diploid chromosome count of 48, indicating they are not true stem cells due to the absence of some pluripotency genes despite expressing POU5f1 and NANOG.
- * When transfected with a specific plasmid, OFEC-17FEN cells displayed strong green fluorescence, highlighting their potential for research and biotechnological applications.
Article Abstract
A new embryonic cell line (OFEC-17FEN) derived from olive flounder was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182235 | PMC |
| http://dx.doi.org/10.12717/DR.2018.22.3.225 | DOI Listing |
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