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Comparative Analysis of the Integument Transcriptomes between Mutant and Wild-Type Silkworms. | LitMetric

Comparative Analysis of the Integument Transcriptomes between Mutant and Wild-Type Silkworms.

Int J Mol Sci

State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, China.

Published: October 2018

AI Article Synopsis

  • The integument in insects offers mechanical support and protection against various external threats, while its diversity relates to factors like body shape and survival.
  • The study analyzed a mutant silkworm that has a rigid, stick-like body, discovering changes in the cuticle's mechanical properties and transcript expression at a specific larval stage.
  • Transcriptome sequencing revealed numerous differentially expressed genes, including those related to chitin and metabolic pathways, providing insights into the genetic mechanisms responsible for the mutant's stiff exoskeleton.

Article Abstract

In insects, the integument provides mechanical support for the whole body and protects them from infections, physical and chemical injuries, and dehydration. Diversity in integument properties is often related to body shape, behavior, and survival rate. The () silkworm is a spontaneous mutant with a stick-like larval body that is firm to the touch and, thus, less flexible. Analysis of the mechanical properties of the cuticles at day 3 of the fifth instar (L5D3) of larvae revealed higher storage modulus and lower loss tangent. Transcriptome sequencing identified a total of 19,969 transcripts that were expressed between wild-type Dazao and the mutant at L5D2, of which 11,596 transcripts were novel and detected in the integument. Differential expression analyses identified 710 upregulated genes and 1009 downregulated genes in the mutant. Gene Ontology (GO) enrichment analysis indicated that four chitin-binding peritrophin A domain genes and a chitinase gene were upregulated, whereas another four chitin-binding peritrophin A domain genes, a trehalase, and nine antimicrobial peptides were downregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that two functional pathways, namely, fructose and mannose metabolism and tyrosine metabolism, were significantly enriched with differentially-expressed transcripts. This study provides a foundation for understanding the molecular mechanisms underlying the development of the stiff exoskeleton in the mutant.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214029PMC
http://dx.doi.org/10.3390/ijms19103158DOI Listing

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