Establishing the quantitative relationship between diffuse speckle contrast analysis signals with absolute blood flow.

Diffuse speckle contrast analysis (DSCA) measures blood flow in deep tissues by taking advantage of the sensitivity of the speckle contrast signal to red blood cells (RBCs) motions. However, there has yet to be presented a clearly defined relationship between the absolute blood flow BF and the measured speckle contrast signal. Here, we derive an expression of linear approximation function for speckle contrast, taking into account both shear-induced diffusive and correlated advective RBCs motions in the vessels. We provide a linear relationship between the slope of this linear function and BF. The feasibility of this relationship is validated by Monte Carlo simulations of heterogeneous tissue with varying vessel radii. Furthermore, based on this quantitative relationship, we can determine the relative contributions of diffusive RBCs motion on the reduction of speckle contrast, considering different vascular morphology and flow profiles.