Lentinus tuber-regium is a sclerotium-forming basidiomycetous mushroom. It has increasingly aroused people's attention for its medicinal effects. In this study, we investigated the applicability of the Agrobacterium tume-faciens-mediated transformation (ATMT) method in L. tuber-regium. A. tumefaciens strain GV 3101 harboring the vector pPEH was used to transform the mycelium of L. tuber-regium strain ACCC50657. The genes for hygromycin B phosphotransferase (hph) and enhanced green fluorescent protein (egfp) under the control of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene promoter of Pleurotus ostreatus were employed as the selection marker and reporter gene, respectively. The optimal cocultivation temperature and time for transformation were 3 days and 4 days at 25°C and 20°C, respectively. Southern blot analysis showed the variation in the copy number and position of hph, which indicated random integration of hph. Polymerase chain reaction and fluorescence microscopy indicated that the P. ostreatus gpd promoter can drive the fused hph-egfp gene expression in L. tuber-regium. This is the first report that the ATMT method was successfully applied to L. tuber-regium. This reliable and efficient transformation method could be a powerful tool for strain genetic improvement and gene function study in L. tuber-regium.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1615/IntJMedMushrooms.2018026991 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative studies on the cultivation, yield, and nutritive value of an edible mushroom, (Rumph. ex Fr.) Singer, grown under different agro waste substrates.
3 Biotech
April 2024
Department of Biotechnology, Vel's Institute of Science, Technology and Advanced Studies (VISTAS), Chennai, TN 600044 India.
In the present study, (Rumph. ex Fr.) Singer collected from Keeriparai forest of Kanyakumari district, South India was cultivated using environmentally benign, low-cost agricultural waste residues (paddy straw, sugarcane bagasse, rice husk, and sawdust) as growth substrates.
View Article and Find Full Text PDFPolysaccharide from mycelium improves DSS-induced colitis in mice by regulating inflammatory cytokines, oxidative stress and gut microbiota.
Food Funct
April 2024
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
(PTR) has been proved to have obvious pharmacological properties. In this study, a polysaccharide was extracted from the mycelium of PTR and administered to DSS-induced colitis mice to clarify the protective effect and mechanism of the PTR polysaccharide (PTRP) on colitis. The results showed that PTRP significantly improved the clinical symptoms and intestinal tissue damage caused by colitis and inhibited the secretion of pro-inflammatory cytokines and myeloperoxidase activity, while the levels of oxidative stress factors in mice decreased and the antioxidant capacity increased.
View Article and Find Full Text PDFFunctional characterization of a catalase gene PtCat associated with sclerotia formation in Pleurotus tuber-regium.
Antonie Van Leeuwenhoek
February 2024
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Article Synopsis
- Pleurotus tuber-regium can form sclerotia to evade oxidative stress, which helps prevent a hyperoxidative state and leads to increased expression of the catalase gene (PtCat).
- Researchers used genetic transformation to create over-expression and interference strains of PtCat to study the relationship between the gene and sclerotia formation.
- While no major differences were noted in the absence of stress, under hydrogen peroxide stress, the over-expression strain showed significantly better growth and catalase activity compared to the interference strain, indicating that catalase plays a key role in sclerotia formation.
Metabolites from a collected in Madagascar.
Nat Prod Res
November 2023
Institute for Sustainable Plant Protection, National Research Council, Torino, Italy.
was isolated from a dead trunk of (Arecaceae) in a lowland moist forest in Antsohihy, Madagascar, and the species was confirmed by molecular analysis and morphological observations. The main bioactive metabolites of the mycelium extracts were identified by mass spectrometry techniques. Five structural diverse metabolites, tryptophol, pyroglutamic acid, prolyldiketopiperazine B, sporol and RKS-1778, were characterised by LC-MS qTOF analysis of the hydro-alcoholic extract.
View Article and Find Full Text PDFMushroom-Related Ethnomycological, Ethnomedical, and Socio-Economic Practices in Nigeria.
Int J Med Mushrooms
August 2023
Crop Science Department, Faculty of Agriculture, Alex Ekwueme Federal University Ndufu-Alike. P.M.B. 1010 Abakaliki, Nigeria.
Article Synopsis
- Native mushrooms are under-used in Africa, particularly in Nigeria, where a study showed widespread consumption but no significant gender differences in foraging practices.
- About 75.6% of interviewees consumed mushrooms, mostly valuing them for their taste, aroma, medicinal properties, and as meat substitutes.
- Women and children are the primary collectors, using mushrooms mainly for treating health issues like skin problems and high blood pressure, with preservation techniques like sun-drying being common.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!