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| http://dx.doi.org/10.1002/mds.27483 | DOI Listing |
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Illuminating understudied kinases: a generalizable biosensor development method applied to protein kinase N.
Commun Biol
January 2025
Department of Medicine, UCSD, La Jolla, CA, USA.
Protein kinases play crucial roles in regulating cellular processes, making real-time visualization of their activity essential for understanding signaling dynamics. While genetically encoded fluorescent biosensors have emerged as powerful tools for studying kinase activity, their development for many kinases remains challenging due to the lack of suitable substrate peptides. Here, we present a novel approach for identifying peptide substrates and demonstrate its effectiveness by developing a biosensor for Protein Kinase N (PKN) activity.
View Article and Find Full Text PDFGlobal Protein Interactome Mapping in Rice Using Barcode-Indexed PCR Coupled with HiFi Long-Read Sequencing.
Adv Sci (Weinh)
January 2025
State Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.
Establishing the protein-protein interaction network sheds light on functional genomics studies by providing insights from known counterparts. However, the rice interactome has barely been studied due to the lack of massive, reliable, and cost-effective methodologies. Here, the development of a barcode-indexed PCR coupled with HiFi long-read sequencing pipeline (BIP-seq) is reported for high throughput Protein Protein Interaction (PPI)identification.
View Article and Find Full Text PDFAI Machine Learning-Based Diabetes Prediction in Older Adults in South Korea: Cross-Sectional Analysis.
JMIR Form Res
January 2025
1, Department of Health Administration, College of Software and Digital Healthcare Convergence, Yonsei University, Changjogwan, Yonseidae-gil 1, Wonju, 26493, Republic of Korea, +82 (0) 33-760-2257.
Background: Diabetes is prevalent in older adults, and machine learning algorithms could help predict diabetes in this population.
Objective: This study determined diabetes risk factors among older adults aged ≥60 years using machine learning algorithms and selected an optimized prediction model.
Methods: This cross-sectional study was conducted on 3084 older adults aged ≥60 years in Seoul from January to November 2023.
Calretinin-expressing neurons in the basal forebrain specifically contact granule cells in the olfactory bulb and modulate odor learning.
J Neurosci
January 2025
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada, K1N 6N5
GABAergic neurons in basal forebrain (BF) nuclei project densely to all layers of the mouse main olfactory bulb (OB), the first relay in odor information processing. However, BF projection neurons are diverse and the contribution of each subtype to odor information processing is not known. In the present study, we used retrograde and anterograde tracing methods together with whole-brain light-sheet analyses, patch-clamp recordings coupled with optogenetic and chemogenetic approaches during spontaneous odor discrimination, and go/no-go odor discrimination/learning tests to characterize the synaptic targets in the OB of BF calretinin-expressing (CR+) GABAergic cells and to reveal their functional implications.
View Article and Find Full Text PDFSingle-cell and spatial transcriptomics illuminate bat immunity and barrier tissue evolution.
Mol Biol Evol
January 2025
Shmunis School of Biomedicine and Cancer Research, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
Bats have adapted to pathogens through diverse mechanisms, including increased resistance - rapid pathogen elimination, and tolerance - limiting tissue damage following infection. In the Egyptian fruit bat (an important model in comparative immunology) several mechanisms conferring disease tolerance were discovered, but mechanisms underpinning resistance remain poorly understood. Previous studies on other species suggested that elevated basal expression of innate immune genes may lead to increased resistance to infection.
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