AI Article Synopsis
- The study focuses on creating 3D models to better understand bone regeneration, aiming to speed up clinical applications and lower research costs.
- The researchers used 3D printing to construct structures from poly(ε-caprolactone) (PCL) that host both stem and endothelial cells in interconnected compartments with a hydrogel matrix.
- The results showed that using strontium ions improved the strength of the hydrogels and enhanced the viability and osteogenic activity of the cells, proving the effectiveness of the design for studying bone regeneration.
Article Abstract
The development of in vitro 3D models to get insights into the mechanisms of bone regeneration could accelerate the translation of experimental findings to the clinic, reducing costs and duration of experiments. This work explores the design and manufacturing of multi-compartments structures in poly(ε-caprolactone) (PCL) 3D-printed by Fused Filament Fabrication technique. The construct was designed with interconnected stalls to host stem cells and endothelial cells. Cells were encapsulated within an optimised gellan gum (GG)-based hydrogel matrix, crosslinked using strontium (Sr) ions to exploit its bioactivity and finally, assembled within compartments with different sizes. Calcium (Ca)-crosslinked gels were also used as control for comparison of Sr osteogenic effect. The results obtained demonstrated that Sr ions were successfully diffused within the hydrogel matrix and increased the hydrogel matrix strength properties under compressive load. The in vitro co-culture of human-TERT mesenchymal stem cells (TERT- hMSCs) and human umbilical vein endothelial cells (HUVECs), encapsulated within Sr ions containing GG-hydrogels and inter-connected by compartmentalised scaffolds under osteogenic conditions, enhanced cell viability and supported osteogenesis, with a significant increase of alkaline phosphatase activity, osteopontin and osteocalcin respect with the Ca-crosslinked GG-PCL scaffolds. These outcomes demonstrate that the design and manufacturing of compartmentalised co-culture of TERT-hMSCs and HUVEC populations enables an effective system to study and promote osteogenesis.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181937 | PMC |
| http://dx.doi.org/10.1038/s41598-018-33472-1 | DOI Listing |
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