Objective: To evaluate the effects of nanoparticle hyperthermia therapy on monocyte function and tumor-derived factors associated with macrophage polarization in a murine osteosarcoma model.
Study Design: Experimental study.
Animals: Female C3H mice.
Methods: Peripheral blood monocyte cell surface phenotype, monocyte chemotaxis, tumor messenger RNA expression, and survival were compared among osteosarcoma (OS)-bearing mice treated with nanoparticle hyperthermia therapy, OS-bearing mice with osteomyelitis, OS-bearing mice, vehicle control mice, and normal control mice.
Results: OS-bearing mice with osteomyelitis had a higher proportion of "nonclassical" monocytes (Ly6C ) compared with all other experimental groups. There were alterations in monocyte expression of multiple chemokine receptors among experimental groups including CXCR2, CCR2, and CXCR4. Monocytes from OS-bearing mice treated with hyperthermia therapy exhibited greater chemotaxis compared with monocytes from OS-bearing mice with osteomyelitis.
Conclusion: OS likely induced alterations in monocyte phenotype and function. Nanoparticle hyperthermia therapy increased in vitro monocyte chemotaxis.
Clinical Impact: Enhancing monocyte/macrophage function in dogs with OS may enhance antitumor immunity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362588 | PMC |
| http://dx.doi.org/10.1111/vsu.12959 | DOI Listing |
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