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The study dealt with etiological diagnostics of bacterial exacerbations of chronic obstructive pulmonary disease (COPD). Samples of bronchial secretion were obtained from 73 patients hospitalized in connection with COPD exacerbations and investigated by the culture method and PSR. The former technique revealed bacterial pathogens in 64 (82%) patients. Gram-positive bacteria (Streptococcus pneumoniae) predominated in patients with stage I and II COPD, Gram-negative (Pseudomonas aeruginosa) and mixt infection in those with stage III and IV COPD. CPR proved more sensitive for isolation of DNA of Gram-negative pathogens (Haemophilus influenzae and Enterobacteriaceae).
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