Studies demonstrate that Tropheryma whipplei (T. whipplei) is present in the lungs of healthy individuals without acute respiratory symptoms or acute respiratory infection and is more common in the lungs of HIV-infected individuals and in smokers. The impact of T. whipplei colonization in the lung on local inflammation and pulmonary dysfunction in HIV-infected individuals is currently unknown. In this study, we performed specific polymerase chain reaction (PCR) and sequencing for T. whipplei in bronchoalveolar lavage (BAL) and induced sputum (IS) samples in 76 HIV-infected participants from three clinical sites. Pulmonary function and proinflammatory cytokine and chemokine levels in BAL were measured. Frequency of T. whipplei in either BAL or IS was 43.4%. The sensitivity and specificity of IS compared to BAL for detection of T. whipplei was 92.3% and 84.2%, respectively, and isolates of T. whipplei in the BAL and IS in the same subject shared genetic identity. Pulmonary function measures were not associated with T. whipplei colonization, and proinflammatory cytokine and chemokine levels in BAL and plasma as well as percentages of inflammatory cells in BAL and IS were not higher in colonized individuals. Overall, these results indicate that T. whipplei colonization in the lung is common, but may not be associated with decreased pulmonary function or inflammation in HIV-infected individuals.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171914 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0205065 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Development of novel broad-range pan-genus PCR assays for the detection of species.
J Med Microbiol
September 2024
Department of Laboratory Medicine and Pathology, Institute of Microbiology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.
Article Synopsis
- A new species related to Whipple's disease was identified in a Belgian patient with pleuritis, prompting the need for tests to detect various species.
- Researchers developed two broad-range pan-genus PCR tests targeting specific genes and tested them alongside existing species-specific PCRs on over 2,600 samples from 2019 to 2022, showing high specificity and decent sensitivity.
- A case of a patient with eye inflammation and lung issues was diagnosed using the new broad-range PCRs, highlighting their role in detecting a potential new lineage of infection when specific PCRs yielded negative results.
Two cases demonstrate an association between Tropheryma whipplei and pulmonary marginal zone lymphoma.
Infect Agent Cancer
July 2024
Institute of Pathology, Cantonal Hospital Baselland, Mühlemattstrasse 11, CH-4410, Liestal, Switzerland.
Background: Marginal zone lymphomas of mucosa-associated lymphatic tissues (MZL of MALT) are a group of indolent B-cell neoplasms, which are thought to arise from chronic antigenic stimulation of B-cells either due to underlying chronic infection or autoimmune disease. Little is known about potential causative pathogens in pulmonary MZL (PMZL), although some data suggests a potential role of Achromobacter (A.) xylosoxidans.
View Article and Find Full Text PDFColonization in Adults and Children: A Prospective Study.
Microorganisms
July 2024
College of Public Health, University of South Florida, Tampa, FL 33620, USA.
We conducted a prospective cohort study at the IRCCS Sacro Cuore Don Calabria Hospital in Negrar di Valpolicella from 2019 to 2021 to investigate the duration of colonization. In addition, the correlation between persistent colonization and the continent of origin, current treatment regimen, clinical manifestations, and parasite coinfection was evaluated. The cohort included subjects who were tested in a previous study (years 2014-2016) and found to be positive for DNA in fecal samples.
View Article and Find Full Text PDFWhipple's disease presenting as weight gain and constipation in a Chinese woman.
BMC Infect Dis
May 2023
Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China.
Background: Whipple's disease is a chronic infection due to Tropheryma whipplei, commonly reported in the Caucasian but not in the Chinese population.
Case Presentation: A 52-year-old female with good past health, was diagnosed with Whipple's disease, presenting with constipation, unintentional weight gain, and fleeting polyarthralgia. Investigations prior to admission showed raised CA125 and computed tomography of the abdomen showed multiple retroperitoneal mesenteric lymphadenopathies.
detection by metagenomic next-generation sequencing in bronchoalveolar lavage fluid: A cross-sectional study.
Front Cell Infect Microbiol
September 2022
Department of Pulmonary and Critical Care Medicine (PCCM), the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.
is the bacterium associated with Whipple's disease (WD), a chronic systemic infectious disease primarily involving the gastrointestinal tract. can also be detected in different body site of healthy individuals, including saliva and feces. Traditionally, has a higher prevalence in bronchoalveolar lavage fluid (BALF) of immunocompromised individuals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!