Photoelectrochemical immunoassay of lipoprotein-associated phospholipase A via plasmon-enhanced energy transfer between gold nanoparticles and CdS QDs/g-CN.

A facile and feasible photoelectrochemical (PEC) immunoassay based on plasmon-enhanced energy transfer between gold nanoparticles (AuNPs) and CdS quantum dots (QDs)/g-CN nanosheets was developed for the ultrasensitive detection of lipoprotein-associated phospholipase A (Lp-PLA). To construct such a sensing platform, the immunosensor was prepared by immobilizing Lp-PLA on a CdS QDs/g-CN-modified electrode. A competitive-type immunoreaction was utilized for Lp-PLA detection, with AuNP-labeled anti-Lp-PLA antibody used as the competitor. Introducing AuNPs with the specific antibody for the antigen target Lp-PLA led to heavy quenching of the photocurrent of CdS QDs/g-CN due to the plasmon-enhanced energy transfer between AuNPs and CdS QDs. The quenching efficiency decreased with increasing target Lp-PLA concentration. Under optimal conditions, the PEC immunosensor presented a good photocurrent response to the target Lp-PLA in the dynamic linear range of 0.01-300 ng mL, with a low detection limit of 5.3 pg mL. Other biomarkers and natural enzymes did not interfere with response of this system. The reproducibility and accuracy of this method for the analysis of human serum specimens were evaluated, and the results given by the method developed here were found to closely correspond to the results obtained with commercial Lp-PLA ELISA kits. Importantly, this protocol offers promise for the development of exciton-plasmon interaction-based PEC detection systems. Graphical abstract ᅟ.