Identification of abnormal scars at their early stage has attracted increasing attentions as the scars can only be assessed qualitatively and subjectively upon maturity, when no invasive procedure is involved. This report introduces a fluorescent probe that targets a potential abnormal scar biomarker (connective tissue growth factor (CTGF) mRNA) in skin fibroblasts. This probe is constructed of hairpin-structured probes (HPs) targeting CTGF mRNA and the nano-graphene oxide (nano-GO) base. The HPs are non-covalently absorbed on the surface of nano-GO, which pre-quenches the fluorescence of HPs. Close proximity of complementary CTGF mRNA would lead to preferential HP hybridization and dissociation from nano-GO, which restores the fluorescence signal from HPs. Utilizing this probe, we can distinguish abnormal fibroblasts derived from abnormal scars and assess the effectiveness of anti-scarring drugs like Repsox and transforming growth factor-beta type I receptor (TGF-βRI) siRNA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.aca.2018.07.016 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Water Extract Inhibits Melanin Synthesis and Promotes Wound Healing.
Life (Basel)
November 2024
MAC Gifu Research Institute, MicroAlgae Corporation, 4-15 Akebono-cho, Gifu 500-8148, Japan.
This study investigated the multifaceted benefits of water extract across various cell lines, including murine B16F1 melanoma cells, human keratinocyte HaCaT cells, and human follicle dermal papilla cells (HFDPCs), to assess its potential in skin health improvement. Initially, the antioxidant capacity of the extract was evaluated using the ABTS assay, revealing significant radical scavenging activity, indicating strong antioxidative properties. Subsequently, extract showed notable inhibition of α-MSH-enhanced melanin production in B16F1 cells without cell toxicity by suppressing tyrosinase expression.
View Article and Find Full Text PDFEffects of linsitinib on M22 and IGF:1-treated 3D spheroids of human orbital fibroblasts.
Sci Rep
January 2025
Departments of Ophthalmology, Sapporo Medical University School of Medicine, S-1 W-16, Chuo-Ku, Sapporo, 060-8543, Japan.
To elucidate the role of IGF1R inhibition in the pathogenesis of Graves' orbitopathy (GO), the effects of linsitinib (Lins) on a recombinant human TSHR antibody (M22) and IGF1 to activate TSHR and IGF1R of human orbital fibroblasts (HOFs) obtained from patients without GO (HOFs) and patients with GO (GHOFs) were studied using in vitro three-dimensional (3D) spheroid models in addition to their 2D planar cell culture. For this purpose, we evaluated 1) cellular metabolic functions by using a seahorse bioanalyzer (2D), 2) physical properties including size and stiffness of 3D spheroids, and mRNA expression of several extracellular matrix (ECM) proteins, their modulators (CCL2 LOX, CTGF, MMPs), ACTA2 and inflammatory cytokines (IL1β, IL6). Administration of IGF1 and M22 induced increases of cellular metabolic functions with the effect on HOFs being much more potent than the effect on GHOFs, suggesting that IGF1R and TSHR of GHOFs may already be stimulated.
View Article and Find Full Text PDFSelenium Enhances the Growth of Bovine Endometrial Stromal Cells by PI3K/AKT/GSK-3β and Wnt/β-Catenin Pathways.
Vet Sci
December 2024
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
The bovine uterus is susceptible to bacterial infections after calving, particularly from (), which often results in endometritis. Additionally, postpartum stress in cows can elevate cortisol levels in the body, inhibiting endometrial regeneration and reducing immune function, thereby further increasing the risk of infection. Selenium (Se) is a common feed additive in dairy farming, known for its anti-inflammatory and antioxidant effects.
View Article and Find Full Text PDFThe interplay between lung galectins and pro-fibrotic markers in post-COVID-19 fibrogenesis: A pilot study.
Life Sci
January 2025
"Aurel Ardelean" Institute of Life Sciences, "Vasile Goldis" Western University of Arad, 310144 Arad, Romania; Department of Histology, Faculty of Medicine, "Vasile Goldis" Western University of Arad, 310144 Arad, Romania. Electronic address:
Aims: COVID-19, caused by the SARS-CoV-2 virus, can lead to serious lung conditions, notably interstitial pulmonary fibrosis.
Main Methods: Our study tracked the progression of fibrosis markers in serial bronchoalveolar lavage (BAL) measurements collected from 16 COVID-19 patients at 1, 3, and 6 months post-infection. Additionally, BAL samples from 10 healthy control subjects were included.
Characterization of the Ocular Phenotype in a Col4a3 Knockout Mouse Model of Alport Syndrome.
Invest Ophthalmol Vis Sci
December 2024
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States.
Purpose: Alport syndrome (AS) is a genetic condition caused by a dysfunctional collagen (IV) α3α4α5 heterotrimer, leading to basement membrane instability and, ultimately, abnormalities in the kidney, inner ear, and eyes. This study aimed to characterize ocular pathology of AS by focusing on inflammatory and fibrotic markers.
Methods: Col4a3tm1Dec knockout (KO) mice eyes were evaluated for the localization of collagen (IV) α3 and collagen (IV) α4, then stained for transforming growth factor-β1 (TGF-β1), TGF-β2, connective tissue growth factor (CTGF), and β-catenin.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!