Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level.

Methods Mol Biol

Faculty of AgriSciences, Department of Molecular Biology and Radiobiology, CEITEC - Central European Institute of Technology, Phytophthora Research Centre, Mendel University in Brno, Brno, Czech Republic.

Published: June 2019

Peptide spectral libraries enable targeted identification and quantitation of low-abundance proteins in a complex plant proteome. Here we describe parallel protein and peptide fractionation techniques to improve plant proteome coverage and facilitate construction of spectral libraries.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-8814-3_6DOI Listing

Publication Analysis

Top Keywords

plant proteome
12
fractionation techniques
8
proteome coverage
8
parallel protein
8
protein peptide
8
spectral libraries
8
techniques increase
4
increase plant
4
coverage combining
4
combining separation
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!