Optical diffraction tomography based on partially coherent illumination (PC-ODT) is a quantitative label-free imaging technique that allows reconstructing of the object's 3D refractive index from measured through-focus intensity images. PC illumination provides advantages such as speckle noise-free imaging and inherent compatibility with conventional wide-field microscopes. Here we experimentally demonstrate that a proper design of the PC illumination, different from the familiar bright-field one, and the use of more realistic optical transfer functions (OTFs) have crucial importance in PC-ODT to significantly increase the accuracy in 3D refractive index reconstruction. While realistic OTFs properly account for the real experimental illumination conditions, the proposed PC illumination design allows for gathering the object spatial-frequency content attenuated when bright-field illumination is used.
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| http://dx.doi.org/10.1364/OL.43.004699 | DOI Listing |
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