AI Article Synopsis
- The study explores how gene expression regulates the proteome in cervical cancer cells by monitoring changes in the transcriptome, translatome, proteome, and RNA-protein interactions.
- It identifies responses to misfolded proteins and oxidative stress, showing that many genes continue to be expressed even during stress, revealing new insights into the unfolded protein response.
- The findings highlight various regulatory pathways and behaviors of genes under stress, emphasizing the complexity of gene regulation in maintaining cell health.
Article Abstract
Maintaining a healthy proteome involves all layers of gene expression regulation. By quantifying temporal changes of the transcriptome, translatome, proteome, and RNA-protein interactome in cervical cancer cells, we systematically characterize the molecular landscape in response to proteostatic challenges. We identify shared and specific responses to misfolded proteins and to oxidative stress, two conditions that are tightly linked. We reveal new aspects of the unfolded protein response, including many genes that escape global translation shutdown. A subset of these genes supports rerouting of energy production in the mitochondria. We also find that many genes change at multiple levels, in either the same or opposing directions, and at different time points. We highlight a variety of putative regulatory pathways, including the stress-dependent alternative splicing of aminoacyl-tRNA synthetases, and protein-RNA binding within the 3' untranslated region of molecular chaperones. These results illustrate the potential of this information-rich resource.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6185107 | PMC |
| http://dx.doi.org/10.7554/eLife.39054 | DOI Listing |
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