In Figure 1b, the GHG sequence motif in the primary microRNA has been moved to the basal stem and the ruler of the basal stem has been shortened to more precisely delineate 11 base pairs. The changes have been made in the HTML and PDF versions of the manuscript.
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http://dx.doi.org/10.1038/s41580-018-0070-6 | DOI Listing |
Front Plant Sci
December 2024
Leibniz University Hannover, Institute of Horticultural Production Systems, Section Woody Plant and Propagation Physiology, Hannover, Germany.
Introduction: The presence of wounds in addition to the excision-induced wounds after severance from the stock plants is known to positively influence adventitious root formation of woody plant cuttings. Previous morphological studies highlighted laser wounding as a technique allowing to precisely control the decisive ablation depth. However, the biochemical processes involved in the response of rooting to the additional wounding remained unexplored.
View Article and Find Full Text PDFFront Bioeng Biotechnol
December 2024
Department of Bioengineering, University of Washington, Seattle, WA, United States.
Colonic epithelium is situated above a layer of fibroblasts that provide supportive factors for stem cells at the crypt base and promote differentiation of cells in the upper crypt and luminal surface. To study the fibroblast-epithelial cell interactions, an crypt model was formed on a shaped collagen scaffold with primary epithelial cells growing above a layer of primary colonic fibroblasts. The crypts possessed a basal stem cell niche populated with proliferative cells and a differentiated, nondividing cell zone at the luminal crypt end.
View Article and Find Full Text PDFSci Rep
December 2024
Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, Republic of Korea.
Small intestinal organoids are similar to actual small intestines in structure and function and can be used in various fields, such as nutrition, disease, and toxicity research. However, the basal-out type is difficult to homogenize because of the diversity of cell sizes and types, and the Matrigel-based culture conditions. Contrastingly, the apical-out form of small intestinal organoids is relatively uniform and easy to manipulate without Matrigel.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
Tea Research Institute, Anhui Academy of Agricultural Sciences, Huangshan, 245000, China.
Background: Adventitious root (AR) formation is the key step for successful cutting propagation of tea plants (Camellia sinensis L.). Studies showed that arbuscular mycorrhizal fungus (AMF) can promote the rooting ability, and auxin pathway in basal stem of cuttings was involved in this process.
View Article and Find Full Text PDFBiotechnol J
December 2024
Institute of Technical Chemistry, Leibniz University Hannover, Hannover, Germany.
The use of optogenetic tools offers an excellent method for spatially and temporally regulated gene and protein expression in cell therapeutic approaches. This could be useful as a concomitant therapeutic measure, especially in small body compartments such as the inner ear, for example, during cochlea implantation, to enhance neuronal cell survival and function. Here, we used the blue light activatable CRY2/CIB system to induce transcription of brain-derived neurotrophic factor (BDNF) in human cells.
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