AI Article Synopsis
- Respiratory syncytial virus (RSV) is a major cause of respiratory infections, particularly challenging to detect in vulnerable populations due to its low viral load.
- Researchers developed a highly sensitive assay called OTNRT-PCR that is easier to use and reduces contamination risks compared to traditional methods.
- The study found that OTNRT-PCR detects RSV more accurately than conventional qRT-PCR, confirming 143 samples that were previously considered negative.
Article Abstract
Respiratory syncytial virus (RSV) causes serious respiratory tract infection worldwide. The relatively low RSV load makes it difficult to detect in frail, elderly, and severely immune-compromised patients. In the present study, we developed a locked nucleic acid--based 1-tube nested real-time RT-PCR (OTNRT-PCR) assay with the advantages of extremely high sensitivity, facile operability, and less likelihood of cross-contamination. The sensitivity, specificity, and clinical performance of the OTNRT-PCR assay were compared in parallel with a conventional TaqMan probe-based real-time PCR (qRT-PCR) assay and a traditional 2-step nested RT-PCR assay. The limit of detection of the OTNRT-PCR assay was 1.02 × 10 TCID50/mL, equivalent to the traditional 2-step nested RT-PCR assay and 25-fold lower than the qRT-PCR assay. Of 616 nasopharyngeal aspirates tested, 143 RSV-negative samples by qRT-PCR were confirmed as positive by sequencing the OTNRT-PCR products. We therefore conclude that OTNRT-PCR is more sensitive than qRT-PCR for detection of RSV in clinical samples.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126397 | PMC |
| http://dx.doi.org/10.1016/j.diagmicrobio.2018.09.001 | DOI Listing |
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