Four phototropin genes (PHOTA1, PHOTA2, PHOTB1, PHOTB2) have been isolated in the moss Physcomitrella patens. These genes encode phototropins that mediate blue-light-induced chloroplast movement. However, the individual functions of these phototropins, including the function of mediating blue-light-induced phototropism, remain unclear. To elucidate the individual functions of P. patens phototropins, each of these phototropin genes was expressed in a phototropin-deficient mutant of Arabidopsis (phot1-5 phot2-1). In addition, fluorescence of GFP fused to these phototropins was examined to determine the subcellular localization of each phototropin. Our results demonstrate that all four P. patens phototropins mediate blue-light-induced phototropism and are associated with the plasma membrane in Arabidopsis. Abbreviations GFP: green fluorescent protein; Pp_phot: Physcomitrella patens phototropin.
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| http://dx.doi.org/10.1080/15592324.2018.1525995 | DOI Listing |
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