Effect of glutamine on heat-shock protein beta 1 () expression during myogenic differentiation in bovine embryonic fibroblast cells.

The objective of this study was to examine the effects of glutamine on heat-shock protein beta 1 () expression in bovine embryonic fibroblast cells during myogenesis. First, to elucidate the role of glutamine on expression during myogenesis, we treated with glutamine in myogenic lineage determinant (MyoD) over-expressed bovine embryonic fibroblast cells (BEFS-MyoD cells). Second, knockdown of using small interference RNA was performed to evaluate whether muscle development by glutamine is dependent on in BEFS-MyoD cells. As a result, glutamine promoted the mRNA level of , , , and mTOR as well as myotube formation, and protein synthesis ( < 0.05). The inhibition of expression during myogenesis has shown to repress the expression of myogenic marker genes (MyoD, , ) ( < 0.01), formation of myotubes and protein synthesis ( < 0.05). According to the results, it is concluded that glutamine regulates expression during myogenesis.