AI Article Synopsis
- The study focused on enhancing the antimicrobial activity of AG-P1441 by optimizing its nutritional requirements using statistical experimental designs.
- Key nutrients identified for effective antimicrobial productivity included glucose, corn starch, and soybean meal, with the best results achieved at a 3% glucose concentration.
- Further optimization in a 5-L bioreactor using a specific medium composition significantly increased antimicrobial activity, evidenced by a 29 mm inhibition zone in the paper disc assay.
Article Abstract
The nutritional requirements for antimicrobial activity of AG-P1441 were optimized using statistically-based experimental designs at a flask level. Based on a one-factor-at-a-time (OFAT) approach, glucose, corn starch and soybean meal were identified as the carbon and nitrogen sources having a significant effect on antimicrobial productivity. As a result of investigating the effect of glucose concentration, the highest antimicrobial activity was observed at 3% concentration. Response surface methodology (RSM) was then applied to optimize the growth medium components (corn starch, soybean meal, MgCl and glutamate). Antimicrobial productivity increased sharply when the medium consisted of 3% glucose, 3.5% corn starch, 2.5% soybean meal, 1.2 mM MgCl and 5.9 mM glutamate. The fermentation using optimized culture medium in a 5-L bioreactor allowed a significant increase in antimicrobial activity, evaluated by the paper disc assay, revealed a 29 mm inhibition zone diameter against .
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6049630 | PMC |
| http://dx.doi.org/10.1007/s10068-017-0257-1 | DOI Listing |
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