A novel process for obtaining phenylpropanoic acid precursor using with a constitutive expression system.

Phenylpropanoids are widely used in food supplements, pharmaceuticals, and cosmetics with diverse benefits to human health. -cinnamic acid or -coumaric acid is usually used as the starting precursor to produce phenylpropanoids. Synthetic bioengineering of microbial cell factories offers a sustainable and flexible alternative method for obtaining these compounds. In this study, a constitutive expression system consisting of phenylalanine/tyrosine ammonia lyase was developed to produce a phenylpropanoic acid precursor in . To improve -cinnamic acid and -coumaric acid production, BioBrick optimization was investigated, causing a 7.2- and 14.2-fold increase in the yield of these compounds, respectively. The optimum strain was capable of producing 78.81 mg/L of -cinnamic acid and 34.67 mg/L of -coumaric acid in a shake flask culture. The work presented here paves the way for the development of a sustainable and economical process for microbial production of a phenylpropanoic acid precursor.