AI Article Synopsis
- Vanadate plays an important role in the growth of the edible fungus Coprinus comatus for medical applications, with a safe concentration established at 3.3 mM.
- During growth at this concentration, the mycelium's biomass yield matches that of the control, despite the potential toxicity of vanadate.
- Spectroscopic studies indicate that vanadate influences sugar phosphate levels and metabolic changes, but higher concentrations become toxic, limiting the fungus's ability to absorb vanadate compared to vanadyl.
Article Abstract
Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.
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| http://dx.doi.org/10.1016/j.jtemb.2018.07.017 | DOI Listing |
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