Induction of mutations in bacteria by a fragment of DNA from herpes simplex virus type 1.

A bacterial assay was developed for the study of mutagenesis by DNA of herpes simplex viruses. The histidine mutations from two of the Ames mutagenesis tester strains were recombined into the Salmonella histidine operon of the F'8 plasmid and each was transferred to a derivative strain of E. coli C from which the resident histidine operon had been deleted. One tester strain could be reverted by a chemical mutagen which induces frameshift mutations and the other could be reverted by a mutagen which induces base-pair substitution mutations. The BamHI G fragment of herpes simplex virus type 1 was cloned in each orientation into the BamHI site of the expression vectors pUC7, pUC8 and pUC9 and were introduced into the new strains of E. coli. The pUC9 plasmid carrying the BamHI G fragment of herpes simplex virus type 1 with the G-E' site closest to the lac promoter showed a higher rate of reversion in the frameshift strain, which varied up to 39-fold greater than the background rate. Since many mutagens are carcinogenic these data suggest the existence of a mutagenic peptide of herpes simplex virus type 1 which might be involved in cell transformation.