Simultaneous Determination of Abscisic Acid and Its Catabolites by Hydrophilic Solid-Phase Extraction Combined with Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry.

An efficient and selective pretreatment method of one-step hydrophilic interaction chromatography-based solid phase extraction (HILIC SPE) was developed using silica as the sorbent to quickly and sensitively detect endogenous ABA and its five catabolites in fresh Oryza sativa tissues. The extracted analytes were sensitively quantified with ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Under the optimized conditions, good linearity of the developed analytical method was obtained in the range of 0.2-1000 ng/mL with linear correlation coefficients ( r) greater than 0.9987. The limits of detection (LODs, signal/noise = 3) ranged from 0.01 to 0.74 ng/mL. The relative recoveries were between 83.3% and 112.0% with the relative standard deviations (RSDs) ranging from 0.5 to 15.0%. Using the proposed method, the concentration variations of ABA and its catabolites were monitored in the salt-stressed rice tissues.