Soil and litter metaproteomics, assigning soil and litter proteins to specific phylogenetic and functional groups, has a great potential to shed light on the impact of microbial diversity on soil ecosystem functioning. However, metaproteomic analysis of soil and litter is often hampered by the enormous heterogeneity of the soil matrix and high concentrations of humic acids. To circumvent these challenges, sophisticated protocols for sample preparation have to be applied. This chapter provides the reader with detailed information on well-established protocols for protein extraction from soil and litter samples together with protocols for further sample preparation for subsequent MS analyses.
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State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. Electronic address:
In farmland shelterbelt systems, the decomposition and/or apoptosis of forest fine root litter could affect farmland soil properties at the tree-crop interface, particularly the soil nitrogen (N) cycling. However, how fine root litter affect the ammonia (NH) and nitrous oxide (NO) losses from farmland soil and the crop production is little known. A soil column experiment covering a whole rice season was conducted to evaluate the dynamics aforesaid in response to fine root litter of Populus (RP) and Metasequoia glyptostroboides (RM) with 0 and 240 kg ha N fertilizer input.
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State Key Laboratory of Urban and Regional Ecology, Research Center for eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
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