Abnormal lysosomal pH is closely associated with many diseases, and real-time monitoring of lysosomal pH is important for understanding the lysosome physiological nature. Here, we present a novel lysosome-targeting two-photon fluorescent probe (MP-lys) for monitoring pH changes in living cells. As a dual site-controlled probe, MP-lys employed morpholine and piperazine groups as the lysosome-targeting groups and pH response sites. MP-lys showed rapid, reversible and sensitive fluorescence response to pH. MP-lys possessed lysosome-targeting properties, and could be used for two-photon imaging of chloroquine-induced pH variation in living cells.
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