Haplocladium microphyllum moss samples were collected in Nanchang, China. Free amino acid (FAA) concentrations and N isotope compositions (δN) in the samples were determined and compared with the bulk N concentrations and δN values. The aim was to determine whether δN values in moss (which are very variable) indicate the sources of atmospheric N. The δN values among individual FAA varied widely (from -19.3‰ to +16.1‰), possibly because of the different sources of N and isotope fractionation in amino acids metabolic pathways. Total N-enrichment for the individual FAAs was equal to total N-depletion relative to δN. The concentration-weighted mean δN value for total FAAs (TFAA) (δN) was -3.1‰ ± 3.2‰, which was similar to δN (-4.0‰ ± 2.9‰). We concluded that a N isotope balance occurred during amino acid metabolism and that little isotope disparity occurred between the concentration-weighted TFAA and bulk N. We concluded that δN ≈ δN ≈ δN. The mean δN (-4.1‰), δN (-4.2‰), and δN (-4.0‰) were similar to the mean δN, which we attributed to little isotope fractionation occurring during their in situ the metabolic pathways. This suggests that δN, δN, and δN in moss can be used to indicate the sources of atmospheric N deposition.
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