AI Article Synopsis
- Researchers isolated a strain of Bacillus amyloliquefaciens, named C5, from olive tree roots, which produces antibacterial biosurfactant lipopeptides.
- They optimized its antibacterial activity using grape seed flour (GSF) at different concentrations, finding 0.2% GSF yielded the best growth and antimicrobial results, with an MIC of 60 μg/ml.
- Thin layer chromatography and MALDI-TOF/MS analyses indicated the production of multiple lipopeptides, including surfactin and bacillomycin D, highlighting GSF as an effective and affordable medium supplement for biosurfactant production.
Article Abstract
An endophytic Bacillus amyloliquefaciens strain called C5, able to produce biosurfactant lipopeptides with a broad antibacterial activity spectrum, has been isolated from the roots of olive tree. Optimization of antibacterial activity was undertaken using grape seed flour (GSF) substrate at 0.02, 0.2, and 2% (w/v) in M9 medium. Strain C5 exhibited optimal growth and antimicrobial activity (MIC value of 60 μg/ml) when incubated in the presence of 0.2% GSF while lipopeptide production culminated at 2% GSF. Thin layer chromatography analysis of lipopeptide extract revealed the presence of at least three active spots at Rf 0.35, 0.59, and 0.72 at 0.2% GSF. Data were similar to those obtained in LB-rich medium. MALDI-TOF/MS analysis of lipopeptide extract obtained from 0.2% GSF substrate revealed the presence of surfactin and bacillomycin D. These results show that GSF could be used as a low-cost culture medium supplement for optimizing the production of biosurfactants by strain C5.
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| http://dx.doi.org/10.1007/s12010-018-2885-1 | DOI Listing |
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