AI Article Synopsis
- Selenoneine, a selenium analog of ergothioneine, is believed to have health benefits, particularly as an antioxidant.
- Researchers are looking into biosynthetic methods to produce pure selenoneine, as its chemical synthesis is difficult.
- This study successfully biosynthesized and isolated selenoneine from genetically modified fission yeast, indicating that this approach could be scaled up for larger production in the future.
Article Abstract
Selenoneine, a naturally occurring form of selenium, is the selenium analogue of ergothioneine, a sulfur species with health relevance not only as a purported antioxidant but likely also beyond. Selenoneine has been speculated to exhibit similar effects. To study selenoneine's health properties as well as its metabolic transformation, the pure compound is required. Chemical synthesis of selenoneine, however, is challenging and biosynthetic approaches have been sought. We herein report the biosynthesis and isolation of selenoneine from genetically modified fission yeast Schizosaccharomyces pombe grown in a medium containing sodium selenate. After cell lysis and extraction with methanol, selenoneine was purified by three consecutive preparative reversed-phase HPLC steps. The product obtained at the mg level was characterised by high resolution mass spectrometry, NMR and HPLC/ICPMS. Biosynthesis was found to be a promising alternative to chemical synthesis, and should be suitable for upscaling to produce higher amounts of this important selenium species in the future.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c8mt00200b | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cracking susceptibility of full-sibs of a cross of a cracking tolerant and cracking susceptible sweet cherry: Relation to cuticle characteristics, microcracking and calcium.
PLoS One
January 2025
INRAE, Biologie du Fruit et Pathologie, Université de Bordeaux, UMR 1332, Villenave d'Ornon, France.
Rain cracking compromises quality and quantity of sweet cherries worldwide. Cracking susceptibility differs among genotypes. The objective was to (1) phenotype the progeny of a cross between a tolerant and a susceptible sweet cherry cultivar for cuticle mass per unit area, strain release on cuticle isolation, cuticular microcracking and calcium/dry mass ratio and (2) relate these characteristics to cracking susceptibilities evaluated in laboratory immersion assays and published multiyear field observations.
View Article and Find Full Text PDFDiverse plant RNAs coat leaves and are distinct from apoplastic RNAs.
Proc Natl Acad Sci U S A
January 2025
Department of Biology, Indiana University, Bloomington, IN 47405.
Transgenic expression of a double-stranded RNA in plants can induce silencing of homologous mRNAs in fungal pathogens. Although such host-induced gene silencing is well documented, the molecular mechanisms by which RNAs can move from the cytoplasm of plant cells across the plasma membrane of both the host cell and fungal cell are poorly understood. Indirect evidence suggests that this RNA transfer may occur at a very early stage of the infection process, prior to breach of the host cell wall, suggesting that silencing RNAs might be secreted onto leaf surfaces.
View Article and Find Full Text PDFInvestigation of alpha-glucosidase inhibition activity of Artabotrys sumatranus leaf extract using metabolomics, machine learning and molecular docking analysis.
PLoS One
January 2025
Research Centre for Plant Conservation, Botanic Gardens and Forestry, National Research and Innovation Agency, Bogor, Indonesia.
One way to treat diabetes mellitus type II is by using α-glucosidase inhibitor, that will slow down the postprandial glucose intake. Metabolomics analysis of Artabotrys sumatranus leaf extract was used in this research to predict the active compounds as α-glucosidase inhibitors from this extract. Both multivariate statistical analysis and machine learning approaches were used to improve the confidence of the predictions.
View Article and Find Full Text PDFNicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway.
Elife
January 2025
Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Cigarette smoking is a well-known risk factor inducing the development and progression of various diseases. Nicotine (NIC) is the major constituent of cigarette smoke. However, knowledge of the mechanism underlying the NIC-regulated stem cell functions is limited.
View Article and Find Full Text PDFExtracellular phospholipase, protease and hemolysin production by strains of opportunistic yeasts from the excreta of Mew Gulls breeding in natural and urban habitats.
Vet Res Commun
January 2025
Soil Science Faculty, Lomonosov Moscow State University, Moscow, 119234, Russia.
Extracellular hydrolytic activity (phospholipase, protease and hemolysin production) was evaluated in 178 strains of potentially pathogenic ascomycetous (Candida parapsilosis, Candida tropicalis) and basidiomycetous (Rhodotorula mucilaginosa) yeasts isolated from the excreta of Mew Gulls. Two bird colonies, one nesting in a natural habitat and the other in an urban habitat at the landfill, were studied simultaneously during their 7-month breeding season. Significant differences in phospholipase and protease production were found between natural and anthropophized strains.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!