The data presented here corresponds to the research paper "Simultaneous detection of invasive signal crayfish, endangered white-clawed crayfish and the crayfish plague using environmental DNA". A crayfish-specific assay was designed and optimised using three real-time PCR supermixes (SYBR™ Green, SsoFast™ EvaGreen® and HOT FIREPol® EvaGreen®). Diagnostic high resolution melt (HRM) data from direct application of assay on both eDNA water samples and field samples from four catchments (two in Wales, two in England) is presented in this article, displaying positive HRM profiles for invasive signal crayfish ( native white-clawed crayfish () and crayfish plague causal agent ().
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141759 | PMC |
http://dx.doi.org/10.1016/j.dib.2018.05.134 | DOI Listing |
J Vet Res
December 2024
Faculty of Fisheries, Akdeniz University, 07070 Antalya, Turkey.
Introduction: Crayfish plague is considered the most important crayfish disease globally. It is caused by the fungus-like agent, . This study aimed to identify and determine the prevalence of using PCR in narrow-clawed crayfish () populations from across Türkiye.
View Article and Find Full Text PDFAnimals (Basel)
December 2024
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, SS, Italy.
We adopted a morphometric approach to provide statistical support for the description of two different morphotypes (I, reproductive, II, non-reproductive) firstly observed in specimens caught in a population from Sardinia Island (western Mediterranean). The morphometric study was preceded by molecular taxonomic identification using the mitochondrial Cytochrome C Oxidase subunit I (COI) gene. The presence or absence of the pathogen , responsible for the plague, was also investigated using the ribosomal Internal Transcribed Spacer (ITS) marker.
View Article and Find Full Text PDFFish Shellfish Immunol
October 2023
LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberg Biodiversity and Climate Research Centre, Georg-Voigt-Str. 14-16, 60325, Frankfurt am Main, Germany.
PeerJ
October 2024
Crayfish Research Centre, Institute for Advanced Environmental Research, West University of Timisoara, Timisoara, Romania.
Ecol Evol
October 2024
Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences University of Helsinki Helsinki Finland.
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