This work provides a protocol for the production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0-36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0-10 h in a previous study.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143716 | PMC |
| http://dx.doi.org/10.1016/j.dib.2018.08.120 | DOI Listing |
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