AI Article Synopsis
- Human pluripotent stem cells can be transformed into primordial germ cell-like cells (hPGCLCs), but achieving mature germ cells has been challenging until now.* -
- In a study, hPGCLCs were cultured in mouse embryonic ovarian somatic cells for about 4 months, successfully differentiating into oogonia-like cells with significant epigenetic changes.* -
- The research indicates that these derived oogonia have the potential for meiotic recombination and showcases the germline capability of human stem cells, marking progress toward human in vitro gametogenesis.*
Article Abstract
Human in vitro gametogenesis may transform reproductive medicine. Human pluripotent stem cells (hPSCs) have been induced into primordial germ cell-like cells (hPGCLCs); however, further differentiation to a mature germ cell has not been achieved. Here, we show that hPGCLCs differentiate progressively into oogonia-like cells during a long-term in vitro culture (approximately 4 months) in xenogeneic reconstituted ovaries with mouse embryonic ovarian somatic cells. The hPGCLC-derived oogonia display hallmarks of epigenetic reprogramming-genome-wide DNA demethylation, imprint erasure, and extinguishment of aberrant DNA methylation in hPSCs-and acquire an immediate precursory state for meiotic recombination. Furthermore, the inactive X chromosome shows a progressive demethylation and reactivation, albeit partially. These findings establish the germline competence of hPSCs and provide a critical step toward human in vitro gametogenesis.
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| http://dx.doi.org/10.1126/science.aat1674 | DOI Listing |
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