It is of the significant importance to achieve facile and on-site detection of heavy metal ions due to the serious harm to environment and human health. Herein, a facile and portable strategy was developed for detection of Hg via portable pressure meter. Biotinylated DNA1 was conjugated on the surface of streptavidin-coated magnetic beads (MBs) to form MBs-DNA1 complex. In the presence of Hg, MBs-DNA1 can hybridize with platinum nanoparticles (PtNPs)-functionalized DNA2 (DNA2-PtNPs) via T-Hg-T binding. Then, PtNPs effectively catalyzed the decomposition of HO to generate oxygen, leading to an increase in pressure of sealed well of 96-well plate. The gas pressure was linearly related with the concentration of Hg in the range between 10 pM and 100 nM with a detection limit of 2.79 pM, which is more sensitive than most of the previous reports. The specific T-Hg-T binding made it easy to selectively detect Hg even when other metal ions co-existed with Hg. Therefore, it offers a cost-effective, rapid, facile and portable way to detect Hg by combining gas-generation reaction with T-Hg-T binding, which holds great potential for detecting Hg in water sample.
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