We investigate the effect of ultrafast laser surface machining on a monocrystalline synthetic diamond sample by means of pulsed Bessel beams. We discuss the differences of the trench-like microstructures generated in various experimental conditions, by varying the beam cone angle, the energy and pulse duration, and we present a brief comparison of the results with those obtained with the same technique on a sapphire sample. In diamond, we obtain V-shaped trenches whose surface width varies with the cone angle, and which are featured by micrometer sized channels having depths in the range of 10-20 μm. By laser writing crossed trenches we are also able to create and tailor on the diamond surface pillar-like or tip-like microstructures potentially interesting for large surface functionalization, cells capturing and biosensing.
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http://dx.doi.org/10.1038/s41598-018-32415-0 | DOI Listing |
J Biomed Mater Res A
January 2025
Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland.
Bone tissue regeneration can be affected by various architectonical features of 3D porous scaffold, for example, pore size and shape, strut size, curvature, or porosity. However, the design of additively manufactured structures studied so far was based on uniform geometrical figures and unit cell structures, which often do not resemble the natural architecture of cancellous bone. Therefore, the aim of this study was to investigate the effect of architectonical features of additively manufactured (aka 3D printed) titanium scaffolds designed based on microtomographic scans of fragments of human femurs of individuals of different ages on in vitro response of human bone-derived mesenchymal stem cells (hMSC).
View Article and Find Full Text PDFMass Spectrom (Tokyo)
December 2024
Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu-City, Toyama 939-0398, Japan.
Matrix-assisted laser desorption/ionization (MALDI), surface-assisted laser desorption/ionization (SALDI), and time-of-flight mass spectrometry (TOFMS) imaging are used for visualizing the spatial distribution of analytes. Mass spectrometry (MS) imaging of a sample with a rough surface with a uniform distribution of an analyte does not provide uniform ion intensities in the image. A shift in the value of the analyte ions is also observed.
View Article and Find Full Text PDFInt J Nanomedicine
January 2025
State Key Laboratory of Ophthalmology, Optometry and Visual Science, National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.
Background: Developing carrier-free nanomedicines via self-assembly of two antitumor drug molecules is a potential strategy for enhancing the combination treatment of tumors. Similarly, conventional chemotherapy combined with photodynamic therapy may synergistically improve the antitumor effect while minimizing the adverse reactions associated with antitumor treatment. Hyaluronic acid (HA) can bind to overexpressed HA receptors on the tumor cell surface, increasing cell internalization and resulting in good tumor-targeting properties.
View Article and Find Full Text PDFDuring type 1 diabetes (T1D) progression, beta cells become dysfunctional and exhibit reduced first-phase insulin release. While this period of beta cell dysfunction is well established, its cause and underlying mechanism remain unknown. To address this knowledge gap, live human pancreas tissue slices were prepared from autoantibody- negative organ donors without diabetes (ND), donors positive for one or more islet autoantibodies (AAb+), and donors with T1D within 0-4 years of diagnosis (T1D+).
View Article and Find Full Text PDFPeerJ
January 2025
Department of Organismal Biology, Uppsala University, Uppsala, Sweden.
In this study, we attempt to illustrate fossil vertebrate dental tissue geochemistry and, by inference, its extent of diagenetic alteration, using quantitative, semi-quantitative and optical tools to evaluate bioapatite preservation. We present visual comparisons of elemental compositions in fish and plesiosaur dental remains ranging in age from Silurian to Cretaceous, based on a combination of micro-scale optical cathodoluminescence (CL) observations (optical images and scanning electron microscope) with minor, trace and rare earth element (REE) compositions (EDS, maps and REE profiles), as a tool for assessing diagenetic processes and biomineral preservation during fossilization of vertebrate dental apatite. Tissue-selective REE values have been obtained using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), indicating areas of potential REE enrichment, combined with cathodoluminescence (CL) analysis.
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