A high-affinity phosphodiesterase, termed PDE II, has been purified about 1400-fold from rabbit skeletal muscle. This enzyme is activated by treatment with proteases. It is also activated specifically by polyarginine and arginine-rich histones, but not by other polyanions. The activation is counteracted nonspecifically by polycations, such as heparin and chondroitin sulphate. When the enzyme is fully activated by polyarginine it is no longer susceptible to activation by proteases. A conformational or structural change must thus occur in the enzyme by the binding of the polyanions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1139/o86-124 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Decoration of Autophagy Detecting Nanoparticle with an Anionic Fluorochrome Enhances Multispectral Characterization of Autophagosome Location and Flux.
Small
December 2024
Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02129, USA.
Autophagy is a key biological process that has proven extremely difficult to detect noninvasively. To address this, an autophagy detecting nanoparticle (ADN) was recently developed, consisting of an iron oxide nanoparticle decorated with cathepsin-cleavable arginine-rich peptides bound to the near-infrared fluorochrome Cy5.5.
View Article and Find Full Text PDFPoly-l-arginine promotes ferroptosis in asthmatic airway epithelial cells by modulating PBX1/GABARAPL1 axis.
Int J Biol Macromol
January 2025
Department of Geriatric Respiratory and Critical Care Medicine, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Anhui Geriatric Institute, Hefei, China; Key Laboratory of Respiratory Diseases Research and Medical Transformation of Anhui Province, Hefei, China. Electronic address:
Article Synopsis
- - Eosinophils are key players in asthma inflammation, releasing harmful proteins like major basic protein (MBP) that damage airway cells and contribute to asthma symptoms.
- - Poly-l-arginine (PLA), which mimics MBP, induces a new cell death mechanism called ferroptosis in airway cells by reducing GABARAPL1 levels, which is linked to eosinophil activity in asthma.
- - Targeting the relationship between eosinophils, MBP, and the mTORC1/PBX1/GABARAPL1 signaling pathway could provide new ways to treat asthma by reducing inflammation and preventing cell death in the airway epithelium.
Exploring the Utility of Cell-Penetrating Peptides as Vehicles for the Delivery of Distinct Antimalarial Drug Cargoes.
ChemMedChem
January 2025
Research School of Chemistry, The Australian National University, Canberra, ACT, 2601, Australia.
The devastating impact of malaria includes significant mortality and illness worldwide. Increasing resistance of the causative parasite, Plasmodium, to existing antimalarial drugs underscores a need for additional compounds with distinct modes of action in the therapeutic development pipeline. Here we showcase peptide-drug conjugates (PDCs) as an attractive compound class, in which therapeutic or lead antimalarials are chemically conjugated to cell-penetrating peptides.
View Article and Find Full Text PDFSustained intra-cellular siRNA release from poly(L-arginine) multilayered nanoparticles for prolonged gene silencing.
Expert Opin Drug Deliv
October 2024
Material Science & Engineering, National University of Singapore, Singapore, Singapore.
Article Synopsis
- The study focuses on improving the release of siRNA from nanoparticles (NPs) inside cells, a process that typically results in rapid release, making it hard to achieve sustained effects.
- By using layer-by-layer (LbL) techniques, researchers developed NPs with multiple layers that enable effective siRNA delivery, demonstrating significant gene knockdown (SPARC) over 21 days.
- The results highlight the ability to measure the release of siRNA over time, linking the quantity released to the effectiveness of gene silencing, providing a valuable method for testing new nanocarrier designs.
Near Infrared-Triggered Nitric Oxide-Release Nanovesicles with Mild-Photothermal Antibacterial and Immunomodulation for Healing MRSA-Infected Diabetic Wounds.
Adv Healthc Mater
December 2024
Hebei Key Laboratory of Functional Polymers, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
Bacterial infection-induced excessive inflammation is a major obstacle in diabetic wound healing. Nitric oxide (NO) exhibits significant antibacterial activity but is extremely deficient in diabetes. Hence, a near-infrared (NIR)-triggered NO release system is constructed through codelivery of polyarginine (PArg) and gold nanorods (Au) in an NIR-activatable methylene blue (MB) polypeptide-assembled nanovesicle (Au/PEL-PBA-MB/PArg).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!